One of the most common types of shuttle vectors is the yeast shuttle vector.
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One of the most common types of shuttle vectors is the yeast shuttle vector.
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Almost all commonly used " S . cerevisiae " vectors are shuttle vectors.
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There are also adenovirus shuttle vectors, which can propagate in " E . coli " and mammals.
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Shuttle vectors are frequently used to quickly make multiple copies of the gene in " E . coli " ( amplification ).
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Yeast shuttle vectors have components that allow for replication and selection in both " E . coli " cells and yeast cells.
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However, vectors may also have elements that allow them to be maintained in another organism such as yeast, plant or mammalian cells, and these vectors are called shuttle vectors.
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The yeast component of a yeast shuttle vector includes an autonomously replicating sequence ( ARS ), a yeast centromere ( CEN ), and a yeast selectable marker ( e . g.
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The " E . coli " component of a yeast shuttle vector includes an origin of replication and a selectable marker, e . g . antibiotic resistance, beta lactamase, beta glastosidase.
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Alternatively, if replication of the DNA in different species is desired ( for example, transfer of DNA from bacteria to plants ), then a multiple host range vector ( also termed shuttle vector ) may be selected.
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